Acrylate-based monolith with integrated gold nanoparticles for proteins extraction and separation

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The fabrication of liquid chromatographic columns with variable interaction chemistry (dual, sequential or gradient composition) is a challenge issue although it presents a high potential for separation and extraction purposes. The direct packing of the stationary phase in a column, the usual way for homogeneous phases, results unpractical when heterogeneous materials are used. Moreover, the final stationary phase´ characterization becomes difficult since it should be performed directly on the stationary phase holder. In this context, the easy in-situ synthesis of monolithic materials makes them a good alternative of choice compared to classic phases. The easy derivatization of the monoliths is also a crucial aspect.

In a recent article, accepted for publication in Microchemical Journal, Currivan et al. have proposed an acrylate-based monolith with integrated gold nanoparticles for the successive extraction and separation of target proteins in the same column (1). The proposed column presents a heterogeneous distribution as the gold NPs (used for the preconcentration of proteins) are located in the initial section of the column while the rest of the column (used for the separation of the previously extracted proteins) remains homogeneous.

Special attention should be given to the column fabrication which is described in depth in the article. The authors use a photografting procedure in order to control the place where the desired derivatization reaction takes places. The derivatization involves an amination of the monolithic material which favors the interaction and retention of gold NPs.

The readers are referred to the original article where they will find a deeper discussion of the results, including the synthesis procedures as well as the characterization of the materials.

References:

(1) Production of polymer monolithic capillary columns with integrated gold nanoparticles modified segments for on-capillary extraction. Link to the article

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